Exploring the Impact of Osmoadaptation on Glycolysis Using Time-Varying Reponse-Coefficients

Clemens Kühn[1] (kuehn@molgen.mpg.de)
Elzbieta Petelenz[2] (elzbieta.petelenz@cmb.gu.se)
Bodil Nordlander[2] (bodil.nordlander@cmb.gu.se)
Jörg Schaber[1] (schaber@molgen.mpg.de)
Stefan Hohmann[2] (stefan.hohmann@gu.se)
Edda Klipp[1] (klipp@molgen.mpg.de)

[1] Computational Systems Biology Group, Max Planck Institute for Molecular Genetics, Ihnestrase 63-73, 14195 Berlin, Germany
[2] Department of Cell and Molecular Biology/Microbiology, University of Gothenburg Box 462 SE-405 30 Goeteborg, Sweden


Abstract

We present a model of osmoadaptation in S.cerevisiae based on existing experimental and theoretical work. In order to investigate the impact of osmoadaptation on glycolysis, this model focuses on the interactions between glycolysis and osmoadaptation, namely the production of glycerol and its influence on flux towards pyruvate. Evaluation of this model shows that, depending on initial relations between glycerol and pyruvate production, the increased glycerol production can have a substantial negative effect on the pyruvate production rate. Existing experimental data and a detailed analysis of the model lead to the suggestion of an interaction between activated Hog1 and activators of glycolysis such as Pfk26.

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